Abstract
Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The antimicrobial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, antifungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabolite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.
Highlights
Microbes from extreme environments have attracted considerable attention in recent years
In vitro antagonistic activity Among the different Streptomyces spp isolated from the haloalkaliphilic origin, the Streptomyces spp
Haloalkaliphilic actinomycetes are a kind of extreme environment actinomycetes, which are highly tolerant to high NaCl concentration, as well as high pH which yielding to valuable industrial products including antimicrobials (Cai et al 2009)
Summary
Microbes from extreme environments have attracted considerable attention in recent years. This is primarily due to the secret that they hold about the molecular evolution of life and stability of the macromolecules. Solar salterns are unique hypersaline environments, characterized by their high salt concentration and alkaline pH (Zafrilla et al 2010). They are the important class of microbial resources and important producers of secondary metabolites including antimicrobials. Found to be proficient to produce antimicrobial compounds and halotolerant enzymes, have been reported from the coastal solar salterns (Vasavada et al 2006; Aruljose et al 2011). Actinomycetes are the most economically and biotechnologically valuable prokaryotes (Lam 2006) On the other hand, a great metabolic diversity and biotechnological potential has been found in halophilic and halotolerant microorganisms
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