Anodic Polarographic Determination of Nilvadipine in Dosage Forms and Spiked Human Urine

Abstract

The anodic polarographic behaviour of nilvadipine was studied by using direct-current (DC), polarography and differential-pulse polarography (DPP). Nilvadipine, being a dihydropyridine derivative, exhibits well-defined anodic waves over the whole pH range of the Britton-Robinson buffers (BRb). In BRb of pH = 4, the diffusion-current constant was 6.45 ± 0.07 µA · mM−1. The current-concentration plots are rectilinear over the ranges 1.6–12.8 and 0.2–12.8 µg/ml for DC polarography and DPP, respectively, with minimum detectability of 0.05 µg/ml (1.3 × 10−7 M) in case of the latter technique. The proposed method was successfully applied for determination of the drug in commercial capsules containing the drug, the percentage recoveries being in good agreement with the label claim. Furthermore, the method was applied for the determination of the drug in spiked human urine, the percentage recovery being 96.84 ± 2.83.