Abstract
The wounds healing is a highly complex process with many phases. Countless substances have been used since the remote times with the purpose a simulating the process. Among them the Orbignya phalerata extract was considered as having a stimulating action. The aim of this study is to verify the healing action of the Orbignya phalerata in the healing of skin surgical wounds by a comparative analysis of the alterations obtained in histological and morphologic aspects. Sixty male adult Wistar rats were used. They were distributed in a randomly in two groups of 30 each and, in one group, Orbignya phalerata extract was used intraperitoneally in a dose of 50 mg/Kg in the first day of surgery. The experimental procedure consisted in a circular incision of two centimeters of diameter with metallic punch. In the control group the substance was not used. The rats were killed in the 7th, 14th, 21st days of the post-operative period and submitted to macro and microscopic analysis. The macroscopic one was made with magnifying glass and paquimeter. For the histological analysis, the sheets were processed with Hematoxilina-Eosina (HE) and trichromic of Masson and slides the vascular proliferation, mononuclear cells, polymorphonuclear cells, fibroblastic proliferation, collagen fibers and re-epithelization were assigned. In the macroscopic view the experimental group of seven days had only one animal with small amount of secretion. In the animals of 14 days all of them had good aspect. The ones of 21 days, complete healing was observed in all animals. In the histological analysis, there was no significant difference among groups, except for the 7th day with significant difference in the fibroblastic proliferation and re-epithelization favoring the experimental group. A favorable effect of the aqueous extract of babassu mesocarp was observed in a microscope analysis in the healing process of cutaneous surgical wounds in rats.
Highlights
The wounds healing is a highly complex process with many phases
Aqui foram escolhidos os períodos de sete, 14 e 21 dias porque entre o 3o e o 7o as fases do processo não são muito diferentes, e no 21o dia já existe cicatrização de ferida cirúrgica, podendo permitir comparação fiel
São Luís: Universitária da Universidade Federal do Maranhão
Summary
O mesocarpo da Orbignya phalerata foi obtido a partir do coco maduro. A coleta foi feita quando estavam caindo naturalmente dos cachos. Utilizou-se um artefato de madeira batendo manualmente na parte superior até ruptura das cascas e em seguida, com o auxilio de uma espátula, separou-se o mesocarpo. O material obtido foi espalhado sobre uma bancada durante três dias para secar e a seguir colocado em estufa de secagem em temperatura de 45-50oC durante 24 horas para retirada total da umidade. Para a preparação do extrato aquoso, o pó foi diluído em solução salina para fazer concentração de 50mg/kg. FIGURA 1 – Rato depilado para incisão dorsal com o punch aquoso por via intraperitoneal em dose única de 50mg/kg no 1o dia antes da operação (Figura 2). Foram sacrificados no 7o, 14o e 21o dias do pós-operatório
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