Abstract
Numerous studies using ultrastructural cytochemistry techniques have been published which bring out and characterize the surface membrane of many cell types. However, few of these studies are concerned with the sarcolemma of heart muscle cells. In particular, no detailed study has been reported relating to cytochemical characterization of isolated sarcolemma from human heart muscle. Thus, the present report was designed to observe and characterize the sarcolemmal ultrastructure of human ventricular myocardium with particular emphasis on anionic molecular organization in the membrane surface by means of electron microscopic and cytochemical studies.The present study was carried out on human heart specimens. The infundibular muscles of right ventricle were obtained during open heart surgery for total correction of 10 patients with congenital heart disease (tetralogy of Fallot). The procedures used for the isolation of cardiac sarcolemma were principally based on the method as described by McNamara et al.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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