Abstract
INTRODUCTIONIn this study, we investigated muscle regenerative ability in different age groups of experimental animals.METHODMuscle precursor cells (MPCs) were cultured from mice. In vitro analysis included cell growth assay, quantitative myotube formation. In vivo studies involved cells transfected or not transfected by adenovirus encoding Vascular Endothelial Growth Factor (VEGF). MPCs were injected subcutaneously into mice. After 2 and 4 weeks, the engineered muscle samples were harvested and analyzed.RESULTSThe young mice MPC growth rate and myotube formation were greater than mature mice and old mice MPC. Bioengineered muscle tissues were smaller and less contractile in old mice group. With expressing recombinant VEGF, bioengineered muscles in all age groups had a greater mass, increased neovascularization, and contractile function. Histological analysis of the in vivo engineered muscle tissue stained positive for muscle specific proteins.CONCLUSIONSThis study demonstrates the MPC regenerative possibilities in young and old mice, and augmentation of neovascularization increased muscle mass and strength of contractility of engineered muscle tissues. Funding NIH Ruth L. Kirschstein National Research Service Award Individual Fellowship (F31), the Novartis‐Stiftung, the Roche Research Foundation, GEBERT RÜF STIFTUNG, and CROWN Foundation.
Published Version
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