Abstract
Aim:The aim of this research was to determine the angiogenesis activity of Jatropha curcas latex in cream formulation on CD34 immune expression during wound healing phase in mice skin.Materials and Methods:Amount of 36 2-month-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. All mice were divided into four groups, namely the base cream as control group (A), sulfadiazine 0.1% cream (B), Jatropha curcas latex cream 10% (C), and J. curcas latex cream 15% (D). All groups were treated entire surface of wound. All experiments were performed twice a day for 10 days. Experiments were terminated on days 3, 7, and 10, respectively. The wound healing was assayed in stained histological section in immunohistochemical of the wounds. The CD34 expression was investigated under a microscope.Results:The results showed that the cream from 10% and 15% latex J. curcas revealed moderate immune reaction to CD34 on days 3 and 7 in wound healing of mice skin.Conclusion:We concluded that the cream from 10% and 15% latex J. curcas has potential as angiogenesis activity in wound healing of mice skin.
Highlights
The wound is defined as a raw skin surface and lost its surface epithelial coverage caused by various types of injuries or diseases [1,2]
We concluded that the cream from 10% and 15% latex J. curcas has potential as angiogenesis activity in wound healing of mice skin
Neovascularization or angiogenesis is important for wound healing process
Summary
The wound is defined as a raw skin surface and lost its surface epithelial coverage caused by various types of injuries or diseases [1,2]. It can be damaged or disruption to the normal anatomical structure and function of skin [3]. Wounds that exhibit impaired healing, including delayed acute wounds and chronic wounds, generally have failed to progress through the normal stages of healing [4]. Angiogenesis plays a role in wound healing by forming new blood vessels from preexisting vessels by invading the wound clot and organizing into a microvascular network throughout the granulation tissue [5]. Angiogenesis plays a role in the provision of nutrients and oxygen to the wound area and increases the formation of granulation tissue with its collagen and connective tissue proteins
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