Abstract
Andean potato latent virus (APLV)는 Group IV (+) sense ssRNA viruses, Tymovirus속으로 분류되는 식물 병원성 바이러스로, 주로 감자에 감염되며 국내 관리급 검역바이러스로 지정되어 있다. 본 연구에서는 검역현장에서 APLV를 신속 정확하게 진단할 수 있는 2개의 RT-PCR 프라이머 조합[조합 2 (404 bp)와 23 (501 bp)]과 각각의 nested PCR 조합(<TEX>$404{\rightarrow}259bp$</TEX> 및 <TEX>$501{\rightarrow}349bp$</TEX>)을 선발하였다. 또한, 진단에 양성대조군으로 활용할 수 있는 유전자변형 양성대조구를 개발하여 실험실 오염에 대한 검증이 가능하도록 하였다. 본 연구에서 개발한 PCR 기반 진단시스템은 향후 검역 현장에서 APLV를 검출하여 우리나라 식물검역에 기여할 것이라고 기대된다. Andean potato latent virus (APLV) is a phytopathogenic virus that belongs to the Group IV (+) sense ssRNA viruses of the genus Tymovirus. It mainly infects potatoes and is specified as a controlled quarantine virus in Korea. In this study, two primer sets of RT-PCR and nested PCR [set 2 (<TEX>$404{\rightarrow}259bp$</TEX>) and set 23 (<TEX>$501{\rightarrow}349bp$</TEX>)], were selected, which can rapidly and accurately diagnose APLV in quarantine sites. In addition, a modified-positive control plasmid is development, can possible verification of laboratory contamination in diagnosis of APLV detection. The PCR-base system developed in this study is expected to diagnose APLV and contribute to the plant quarantine in Korea.
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