Anatomical consequences of neonatal infraorbital nerve transection upon the trigeminal ganglion and vibrissa follicle nerves in the adult rat

Abstract

A large body of experimental literature has demonstrated that neonatal infraorbital nerve damage in rodents produces anatomical and/or functional alterations of the normal whisker representation in central trigeminal structures. Less is known about the organization of primary afferent components of the trigeminal system following this manipulation. Such information provides an important basis for interpreting the central changes observed following damage of infraorbital nerve fibers at birth. We have therefore examined the composition and order of peripheral innervation in the pathway from the trigeminal ganglion to the vibrissa follicles in adult rats subjected to unilateral neonatal infraorbital nerve transection. Electron microscopy was used to determine the number and diameter of myelinated and unmyelinated fibers in vibrissa follicle nerves of these animals. Wheat germ agglutinin-horseradish peroxidase and fluorescent retrograde tracers were employed to examine the number and diameter, as well as the topographic organization and branching, of ganglion cells innervating the vibrissae in these rats. The data presented below indicate that neonatal infraorbital nerve transection has the following consequences within the adult trigeminal nerve and ganglion: 1) an alteration of the gross morphology of vibrissal nerves, 2) a significant reduction in the average number (85.4%) and diameter (32.6%) of myelinated, but not unmyelinated, follicle nerve axons, 3) a significant decrease in the average number (36.8%) of trigeminal ganglion cells innervating vibrissa follicles, 4) no significant change in the distribution of ganglion cell diameters, 5) an increase in peripheral branching (1.8-fold) of these ganglion cell axons, and 6) an alteration of somatotopic order within the trigeminal ganglion. Taken together, these data indicate that neonatal infraorbital nerve transection produces a profound reorganization of the primary afferent component of the trigeminal neuraxis.