Abstract
SummaryPurposeTo investigate outer retinal injury in glaucoma.MethodsStudies were done on human glaucomatous eyes as well as eyes from a laser trabecular meshwork destruction model of experimental glaucoma (EG) in non‐human primates (NHPs), including light microscopy and in situ hybridization (ISH) for mRNA using probes specific for rod, S‐cone and M/L‐cone opsins. Choroidal blood flow (ChBF) was measured using non‐recirculating fluorescent microspheres. Functional studies on the NHP eyes included multifocal and full‐field electroretinography (mfERG and ffERG).ResultsSwelling of M/L‐cones) was a common finding in both the glaucomatous human and NHP EG eyes. Cone loss was also observed in some of the human eyes. ISH for mRNA showed reduced levels in both the S‐ and M/L‐cones in human glaucoma and NHP EG. Supranormal mfERG waveforms were a common feature of the NHP EG eyes. ChBF was greatly reduced in eyes with EG. The ffERG in NHP eyes with advanced EG showed larger reductions in photopic than in scotopic a‐ and b‐wave single‐flash responses; particularly at higher intensities.ConclusionMultiple lines of evidence show cone injury in human glaucoma and in NHP EG. Reduced ChBF could be the cause.
Published Version
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