Analyzation of TP53 expression, mutation,codon 72 polymorphism and the relationship with human papillomavirus infection of the Chinese Laryngeal squamous cell carcinoma patients

Abstract

Objective To analyze the expression,mutation and codon 72 polymorphism in the Chinese laryngeal squamous cell carcinoma patients and the relationship with HPV infection.Methods The paraffin-embedded tissue blocks from 64 pathological-diagnosed laryngeal squamous cell carcinoma patients were included in this study.PCR and direct sequencing were used to screen the mutations in TP53 gene and immunohistochemistry was used to evaluate the expressing profiles of p53 proteins.Functional analysis software online were used to analyze the function changes of mutated p53 proteins.The relationship between TP53 mutation and phenotypes of SNP72 with other features of the patients was analyzed by Chisquare and Fisher' s exact test.Results Out of 64 laryngeal squamous cell carcinoma patients,sequencing confirmed 30 TP53 mutations in 22 cases (73.3％) and p53 immunohistochemistry (IHC) confirmed 20 (31.3％) positive staining.Online software analysis showed that most of the mutated p53 proteins lost their normal function.Analyses of SNP72 polymorphism revealed that the patients with Arg phenotype had significantly older onset ages than those with Pro phenotype but without statistic difference.Conclusion TP53 mutations are frequently observed in Chinese patients with laryngeal squamous cell carcinoma and this is one of the pathogenesis for laryngeal squamous cell carcinoma. Key words: Carcinoma, squamous cell; Protein p53; Polymorphism; Papillomavirus, human