ANALYTICAL METHODS TO DETECT THE AUTOOXIDATION OF ADRENOLUTIN AS A STEP IN CATECHOLAMINE METABOLISM

Abstract

The acute hemodynamic effects of organophosphate (OP) intoxication include positive chronotropic and inotropic changes along with increases in intraventricular pressure and coronary blood flow. These observations are consistent with an enhanced sympathetic tone that correlates neurogenic cardiomyopathy to adrenergic overactivity and subsequent, focal catecholamine (CA) release and cellular oxidative stress. Several mechanisms have been proposed to explain the cardiotoxicity associated with elevated CA concentrations. However, it is suggested that the oxidative metabolites of CA's, rather than (or in addition to) the parent CA's per se, may initiate or be in part responsible for the cardiotoxicity. The chromatographic profile of adrenochrome (1) and adrenolutin (2) (Figure 1) are described in this study in an isocratic, reverse phase HPLC method using UV/VIS and electrochemical (EC) detection. The aqueous adrenochrome standard shows a retention time of 1.9 minutes under employed conditions. Furthermore, us...