Analytical method validation of cinnamaldehyde content in cinnamon (Cinnamomum burmannii) extract using high-performance liquid chromatography

Abstract

The use of herbal medicinal plants has been found widely in the community as traditional medicine. One example is the Cinnamon plant (Cinnamomum burmannii) which contains cinnamaldehyde as the main compound. Cinnamaldehyde is known to have a major role in lowering blood sugar levels. Determination of the content of bioactive compounds in extracts is beneficial in determining the safety, quality, and efficacy of the plants used. So it is necessary to do a quantitative analysis test on the cinnamaldehyde compound in cinnamon plants by determining its levels using the HPLC method. It is necessary to validate the method analysis based on parameters including selectivity, linearity, accuracy, precision, detection limit, and quantitation limit. The mobile phases in this method were acetonitrile mobile and 0.04�etic acid solution (60:40), The stationary phase was octadecylsilane (C-18), The flow rate was adjusted at 1.0 mL/min with column temperature adjusted at 29°C, injection volume 20 μl, and using a Photodiode array detector at a wavelength of 280 nm. The results showed that the resolution value obtained was 3.401; the correlation coefficient value obtained is 0.9941; The percent recovery obtained between 98.74% - 101.95%; percent RSD obtained between 0.92% - 2.68%; and the LOD and LOQ values were 0.069 ppm and 0.23 ppm. The HPLC method met the validation requirement and can conclude to be valid.