Abstract
High-performance liquid chromatography (HPLC) has become the most significant analytical technique of the last 30 years. Whereas HPLC has revolutionized analytical chemistry in general, its significance is mostly recognized in the development of modern biochemistry/biotechnology. Indeed, the scientific accomplishments of many biological disciplines can be largely credited to the development of modern HPLC (). The popularity and success of the technique has arisen from synergistic improvements in surface chemistry, column technology, instrumentation, and software developments. HPLC is now used for biopolymer purification at the research level, large-scale purifications, and analysis at the development level. In particular, the efficiency, speed, and recovery accomplished by HPLC allowed never seen development of modern biotechnology and pharmaceutical R&D.
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