Abstract
Objective To isolate and characterize the bioactive secondary metabolites from Aspergillus ochraceus ( A. ochraceus) MP2 fungi. Methods The anti bacterial activity of marine sponge derived fungi A. ochraceus MP2 was thoroughly investigated against antagonistic human pathogens. The optimum inhibitory concentration of the fungi in the elite solvent was also determined. The promising extracts that showed good antimicrobial activity were subjected to further analytical separation to get individual distinct metabolites and the eluants were further identified by GC MS instrumental analysis. The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer (ITS) region of 5.8s rRNA using specific ITS primer. The novelty of the strain was proved by homology search tools and elite sequences was submitted to GENBANK. Results Three bioactive compounds were characterized to reveal their identity, chemical formula and structure. The first elutant was identified as α – Campholene aldehyde with chemical formula C 10 H 16 O and molecular weight 152 Da. The second elutant was identified as Lucenin-2 and chemical formula C 27 H 30 O 16 and molecular weight 610 Da. The third elutant was identified as 6-Ethyloct- 3-yl- 2- ethylhexyl ester with Chemical formula C 26 H 42 O4with molecular weight 418 Da. Conclusions The isolated compounds showed significant antimicrobial activity against potential human pathogens. Microbial secondary metabolites represent a large source of compounds endowed with ingenious structures and potent biological activities.
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