Analysis the effect of different extraction methods towards the successfulness of amplification 24 loci short tandem repeat (STR): Study of forensic samples

Abstract

Science Crime Investigation (SCI) is a criminal investigation based on scientific proves. The utilization of DNA analysis are proven to be more efficient and accurate. DNA typing method was used to identify human based on DNA profile. The technique consisted of DNA extraction, DNA quantification, DNA amplification, and fragment analysis. DNA extraction from the forensic evidence was the crucial step to determine the success rate of the DNA analysis. The aimed of this research was to analyze the effect of different DNA extraction methods that commonly used in the forensic investigation in Indonesia. The DNA extraction methods were: (A) silica based method using QIAamp® DNA Investigator kit (Qiagen), (B) magnet beads based method using Prepfiler® BTA forensic kit (Applied Biosystem), and (C) resin based method Chelex® 100. Biological evidence used in this research were saliva, blood, hair, bone, and touch DNA. The indicators were the number of DNA concentration, inhibitor, and the percentage of amplified allele on 24 loci Short Tandem Repeat (STR). Three indicators were used to compare the effectiveness of DNA extraction methods in particular biological samples. Based on the result, silica-based method using QIAamp® DNA Investigator extraction method was effectively used on saliva, blood, and bone samples. Prepfiler® BTA Forensic kit extraction method was effective on saliva, blood, hair, and semen samples. Chelex®100 extraction methods were effectively used on saliva, blood, and hair samples.