Abstract
Objective:To investigate allergen extract and to seek the main allergens of phoenix roebelenii pollen by the technology of two dimensional electrophoresis(2-DE)in protein analysis and immunoblotting.Method:Phoenix roebelenii pollen allergen extract was prepared with routine method, 2-DE combined with immunoblotting assay(Western Blotting),laser imagescanning, 2-DE gel analysis software were used to analyzing its protein components.Result:About 601 soluble proteins spots were revealed in coomassie stained gels.Most of the proteins had a molecular weight (Mr) of 20 to 130 kD, and an isoelectric point(pI) value of 4.0 to 8.0. Immunoblotting of 2-DE were showed and there were 19 specific antigen spots, compared with the controls.Conclusion:2-DE is a good method in protein analysis of ephemeroptera allergen extract, and it is useful in seeking main allergens and further research for allergic components.
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Schedule a callMore From: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
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