Abstract

In this study, amino acids, proteins, and microbial communities in sludge from different wastewater treatment plants (WWTPs) were analyzed. The results showed that the bacterial communities of different sludge samples were similar at the phylum level, and the dominant bacterial species in sludge samples with the same treatment process were the consistent. The main amino acids in EPS of different layers were different, and the amino acid results of different sludge samples were quite different, but the content of hydrophilic amino acids in all samples was higher than that of hydrophobic amino acids. And the total content of glycine, serine, and threonine related to sludge dewatering was positively correlated with protein content in sludge. In addition, the content of nitrifying bacteria and denitrifying bacteria in sludge was also positively correlated with the content of hydrophilic amino acids. In this study, the correlations between proteins, amino acids, and microbial communities in sludge were analyzed respectively, and the internal relationship was found. And it provided ideas for further study of sludge dewatering characteristics in the future.

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