Abstract
BackgroundDysregulation of microRNAs (miRNAs) plays a critical role during the occurrence and progress of pituitary adenomas (PAs). However, the roles of miRNAs in the invasiveness of PA are poorly understood. This study aims to more comprehensively and specific define the relationship between altered miRNA and PA invasion.MethodsThe differential expression of miRNAs (DEMs) between invasive PAs (IPAs) and non-invasive PAs (NPAs) was explored by RNA sequencing and which functions were analyzed by gene ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG). The miRNA-mRNA network was predicted with bioinformatics.ResultsWe identified 31 upregulated miRNAs and 24 downregulated miRNAs in IPAs compared with NPAs. GO analysis and KEGG pathway analysis showed the DEMs were mainly associated with cell proliferation and cell cycle pathway. In addition, on the count of predicted miRNA-mRNA network, two hub miRNAs were identified.ConclusionsOur results demonstrate the miRNA-mRNA network in detail, which suggest that miRNA may be a promising target in diagnosis and therapy for IPAs.
Highlights
Dysregulation of microRNAs plays a critical role during the occurrence and progress of pituitary adenomas (PAs)
Quality assessment of sequencing data The results showed the patterns of gene expression among the samples were similar, and the Pearson correlation between samples is similar in the two groups (Fig. 1a, b)
Apportionment and annotation of Differential expression miRNA (DEM) After summarizing and classifying the sequence reads into different RNA categories, such as miRNA, sn/ snoRNA, tRNA, and rRNAs, the pie chart is drawn to annotate and classify the total reads
Summary
Dysregulation of microRNAs (miRNAs) plays a critical role during the occurrence and progress of pituitary adenomas (PAs). The roles of miRNAs in the invasiveness of PA are poorly understood. This study aims to more comprehensively and specific define the relationship between altered miRNA and PA invasion. PA is one of the most common intracranial tumors with an incidence of 10–15% [1]. MiR-193b exerts tumor suppressive effects in human acute myeloid leukemia by inducing. In order to better understand the mechanism of invasiveness in PAs, it is necessary to clarify the miRNA regulatory network in IPAs. In this study, we detected DEMs in IPAs and NPAs by RNA sequencing, and established the co-expression network contain miRNAs and predicted target genes by Cytoscape.
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