Abstract
The investigations on sources and viability of stem cells are important as stem cell transplantation has shown promising results in diseases like leukemias and lymphomas. Umbilical cord blood samples were collected in a closed aseptic system. The samples were diluted with phosphate buffered saline, treated with ficol and centrifuged at 15,000 rpm for the recovery of progenitor stem cells.The stem cells were cryopreserved with different media Containing DMSO, patient's serum and human albumin.The viability of the cells was studied by dye exclusion method. Suitable media are necessary for optimal cryoprotection and prevention of cryoinjury DMSO is essential for improved cryopreservation and post-thaw quality. The addition of a protein additive also provides a protective effect. The medium containing DMSO, DMEM and patient's serum proved to be the most effective for cryopreservation and viability as high as 82.4% was achieved after one year. The unique findings of the present study are that the addition of patient's serum enhances the cryoprotection and cord blood stem cells can be stored at -20°C for the duration up to two months instead of the requirement of storage at ultralow temperature at -186°C.
Highlights
Main sources of stem cells are bone marrow, Introduction: In view of the rapidly growing adult peripheral blood, embryos and umbilical potential of stem cell therapies for a number cord blood
The samples were diluted with phosphate buffered saline, treated o with ficol and centrifuged at 15,000 rpm for the recovery of progenitor stem .c cells.The stem cells were cryopreserved with different media Containing l DMSO, patient’s serum and human albumin.The viability of the cells was l studied by dye exclusion method
A rich source of the stem cells.(4) Both term and pre term cord bloods contain significantly Composition of different cryoprotectants higher number of early and committed stem used was as follows: cells.(5) Umbilical cord blood stem cells are more sensitive to ex vivo expansion and less immunoreactive.(6,7) instead of being discarded as a biological waste, the umbilical cord blood can be preserved as a source of stem cells
Summary
Main sources of stem cells are bone marrow, Introduction: In view of the rapidly growing adult peripheral blood, embryos and umbilical potential of stem cell therapies for a number cord blood. The other genetic and degenerative diseases(1,2,3) numbers of stem cells present in adult the investigations on analysis of the sources peripheral blood are too low and large and viability of stem cells are highly relevant. Volumes of blood may be required for Copyright © Journal of Stem cells and Regenerative medicine. Journal of Stem Cells & Regenerative Medicine sufficient numbers of stem cells. There are The cord blood samples were cryopreserved ethical issues associated with the use of with three different media: Medium 1, embryonic stem cells. Present study deals with cryopservation and viability testing of cord blood stem cells using different media and Medium 1 Patient’s serum and DMSO in a ratio of
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