Analysis of the Relationship Between IL-10 Gene Polymorphism and Hypertension Susceptibility in Han Nationality in Jianghan Plain

Abstract

Objective: To analyze the association between interleukin-10 (IL-10) promoter-1082 and -819 polymorphisms and hypertension in Han nationality in Jianghan Plain. Methods: A total of 50 Han patients with hypertension in Jianghan Plain, who were hospitalized in our hospital from March 2018 to April 2018, were selected as the experimental group. 50 healthy Han Chinese people in Jianghan Plain were selected as the control group. After a series of reactions such as genomic DNA extraction and PCR amplification, polymorphisms of IL-10-819 and -1082 were performed using ABI's BAT3.1 sequencing kit. Analysis and analysis of the risk of developing hypertension at each point. Results: There were three genotypes of C/C, C/T and T/T in the IL-10-819 locus. There was no significant difference between the two groups (P>0.05). There were G/G, G/A and A/A genotypes in IL-10-1082 loci in both groups. There was no significant difference between the two groups (P>0.05). Logistic regression analysis showed that compared with IL-10-819C/C genotype, the risk of hypertension in the population carrying IL-10-819C/T and T/T genotypes was OR＝1.19 (95%CI＝0.71~1.05), χ２=0.73, P>0.05, OR＝1.05 (95%CI＝1.01~1.10), χ２=0.96,P>0.05, the difference was not statistically significant (P>0.05). Compared with IL-10-1082A/A, the risk of developing hypertension in the population expressing IL-10-1082G/A and G/G genotypes was OR＝1.2 (95%CI＝0.76~1.12), χ２=0.704, P>0.05, OR＝1.33 (95% CI＝0.77~1.33), χ２=0.97, P>0.05, the difference was not statistically significant (P>0.05). Conclusion: The distribution of genotypes and alleles of IL-10-819 and -1082 loci in Han patients with hypertension and healthy people in Jianghan Plain is similar, and the genotype mutations of IL-10-819 and -1082 are irrelevant with hypertension.