Abstract
The aim of the study was to investigate the effect of laser irradiation of mycelium on the amount of biomass and synthesis of P. ostreatus polysaccharides.Materials and methods. For the study, 6 strains of P. ostreatus from the Collection of cultures of basidiomycetes of the Department of Botany and Ecology of Vasyl Stus DonNU were used. A device consisting of LED lasers was used for laser irradiation of vegetative mycelium: BRP – 3010–5, with red spectrum radiation with a wavelength of 635 nm; BBP – 3010–5 with blue spectrum radiation with a wavelength of 405 nm and BGP – 3010–5 with green spectrum radiation with a wavelength of 532 nm. The level of biomass accumulation was determined by weight. The polysaccharide content was determined by the phenol-sulfur method.Results. The most effective was green light irradiation with a wavelength of 532 nm. For strain P-192 – the amount of biomass increased by 71.4 %. For strains P-191 and P-155 biomass increased by 60 % and 53.5 %. For strains P-108, P-154 and P-6v, the amount of biomass increased from 33.3 to 50 %. For strain P-192, the amount of mycelial endopolysaccharides increased by 42.0 %. For strains P-191 and P-6v, the amount of endopolysaccharides increased by 39.3 % and 38.7 %. For strains P-108, P-155 and P-154 the amount of mycelium endopolysaccharides increased from 30.7 % to 35.8. For strain P-192 the content of exopolysaccharides increased by 30.5 %. For strains P-154 and P-191, the amount of exopolysaccharides increased by 28.1 % and 27.8 %. For strains P-108, P-155 and P-6v the content of exopolysaccharides increased from 24.6 % to 25.8 %.Conclusions. The most effective mode of photoactivation of P. ostreatus mycelium for obtaining target products was determined. In particular, the best response was observed in response to green light with a wavelength of 532 nm for strain P-192 – the amount of biomass increased by 71.4 %, the amount of mycelium endopolysaccharides increased by 42.0 %, and the content of exopolysaccharides increased by 30.5 %
Highlights
Pleurotus ostreatus belongs to the fungi that cause white rot of wood and has a well-developed enzymatic apparatus that is able to absorb a variety of carbon-containing substrates [1, 2]
We found that laser irradiation of vegetative mycelium of P. ostreatus with light of different spectral composition activates mycelial growth, so we hypothesized that the use of photoactivated mycelium will increase the synthesis of biologically active substances and the amount of biomass [6]
Under the action of this irradiation regime, the best response to light was observed for strain P-192 – the amount of biomass increased by 71.4 % according to the control
Summary
Pleurotus ostreatus belongs to the fungi that cause white rot of wood and has a well-developed enzymatic apparatus that is able to absorb a variety of carbon-containing substrates [1, 2]. P. ostreatus attracts the attention of scientists due to its accessibility and ease of use. This allows the introduction of new modern methods of intensification of growth and biochemical parameters of this fungus. This method is laser irradiation of the mycelium, which can significantly improve the growth and development of fungi. It should be noted that the use of helium-neon and argon lasers, which have large dimensions and significant energy consumption, complicates the technology of stimulating the growth and development of fungi [5]. It is much more effective to use LED lasers to intensify the metabolic processes of macromycetes [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
