Abstract
Objective To explore the frequency and function of antigen-specific T cells at different courses of condyloma acuminate(CA)by means of the sHLA-A2/HPV-6 E7 tetramer constructed from HLA-A * 0201 and its restricted peptide HPV-6 E7(22-30).Methods Peripheral blood lymphecytes (PBL)were collected from the HLA-A2 positive individuals without clinical manifestations and those CA cases at different courses.The T2 (HLA-A2+)cells born with HPV-6 E7 were used as the priming cells to co-cultivate with the PBL taken from different courses of the patients to generate the peptide-specific CTL through long term co-cultivation.The HLA-A2/HPV-6 E7 tetramer was labeled with PE in vitro and the proliferated specific CTLs after cultivation were stained with fluorescence by making use of sHLA-A2/HPV-6 E7 tetramer and FITC-CD8 McAb.And the killing activities of these specific CTLs were tested by LDH assay.Results The frequency of specific CTLs in control group was (0.320±0.076)%.The frequencies of specific CTLs in recurrent CA and during remission were ( 1.100±0.064)%and ( 1.115±0.071)%,respectively,both of which were of no significant difference (t=0.34,P=0.74)and higher than that of the control.The killing activities in recurrent CA and during remission were (51.18±0.48)%and (59.33±1.71)%,respectively.The significant difference existed betwcen these two groups.Conclusion The tetramer can be applied directly to visualize antigen-specific CTLs efficiently and become the critical approach in the assessment of T cell immune responses in CA patients.The difference is not obvious in the frequencies of specific CTLs at different courses of CA.But the difference is prominent in the CTLs killing frequency at different courses of CA.The CTLs can be highly activated and eliminate the HPV in remission CA,so the disease can be cured. Key words: Human papillomavirus; E7 antigen; Condyloma acuminata; Tetramer; Cytotoxin T lymphocyte
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