Analysis of the effects of sulfamethoxazole on the secondary metabolites and antioxidants in oilseed rape (Brassica napus L.) and the underlying mechanisms

Abstract

The secondary metabolism of plants is key for mediating responses to environmental stress, but few studies have examined how the relationship between secondary metabolism and the stress response of plants is affected by exposure to antibiotics. Here, we studied the effects of sulfamethoxazole (SMZ) on the secondary metabolism and antioxidant activity of oilseed rape (Brassica napus L.). SMZ significantly affected the growth of rape seedlings. Low and high concentrations of SMZ induced the production of a large number of reactive oxygen species (ROS) in rape seedlings, which damaged cells. SMZ stress altered the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as the content of malondialdehyde (MDA). SMZ promoted the activities of phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), cinnamic acid-4-hydroxylase (C4H), and 4-coumaric acid: coenzyme A ligase (4CL) by activating the phenylpropanoid pathway. The content of secondary metabolites changed. The content of phenolic acids and flavonoids increased, and the content of sinapine and anthocyanins was altered to cope with the oxidative damage induced by antibiotics. Transcriptomic and metabolomic analysis showed that differentially expressed genes and differentially expressed metabolites were mainly involved in Phenylpropanoid biosynthesis. SMZ alters the secondary metabolites of rapeseed, which mitigates the deleterious effects of stress, by modulating upstream secondary metabolism pathways and the production of plant hormones involved in signal transduction. In sum, these results provide a new perspective on the effects of SMZ on plants relative to secondary metabolites and improve our understanding of the toxicity of SMZ.