Analysis of the correlation between severe pneumonia and inflammatory factors and TBX21 locus rs16947078

Abstract

Objective To analyze the correlation between severe pneumonia and inflammatory factors and TBX21 locus rs16947078. Methods From March 2017 to March 2019, in the Fourth People's Hospital of Wenling, 71 patients with severe pneumonia were selected, and 83 patients with general pneumonia were selected during the same period.The levels of interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-18 (IL-18) and tumor necrosis factor-alpha (TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). The genotype and gene distribution of rs16947078 at TBX21 locus were detected by real-time fluorescence quantitative PCR. Results The score of CPIS in the severe pneumonia group was higher than that in the common pneumonia group [(6.57±1.20)points and (4.93±1.05)points] (t=9.045, P<0.05). The serum levels of IL-6 [(65.42±13.25)ng/L], IL-8 [(457.87±45.36)ng/L], IL-18 [(87.59±15.46)ng/L] and TNF-α[(58.98±13.25)ng/L] in the severe pneumonia group were higher than those in the general pneumonia group [(36.71±8.98)ng/L, (218.98±32.45)ng/L, (54.37±12.10)ng/L and (37.54±7.90)ng/L] (t=15.926, 37.959, 14.946, 12.394, all P<0.05). The G distribution of rs16947078 allele at TBX21 locus in the severe pneumonia group (40.85%) was higher than that in the normal pneumonia group (18.07%) (χ2=9.724, P<0.05). The GG (36.62%) of TBX21 locus rs16947078 in the severe pneumonia group was higher than that in the common pneumonia group (12.05%), while the AA (52.11%) of TBX21 locus in the severe pneumonia group was lower than that in the common pneumonia group (72.29%) (χ2=12.898, 6.682, P<0.05). Conclusion The levels of inflammatory factors IL-6, IL-8, IL-18 and TNF-α in patients with severe pneumonia aresignificantly increased, and the TBX21 locus rs16947078 allele A may be a susceptible gene for severe pneumonia. Key words: Pneumonia; Interleukin-6; Interleukin-8; Interleukin-18; Tumor necrosis factor-alpha; TBX21 locus rs16947078; Correlation