ANALYSIS OF THE ASSOCIATION OF RS4977574-POLYMORPHIC VARIANTS OF THE ANRIL GENE WITH THE DEVELOPMENT OF ACUTE CORONARY SYNDROME IN INDIVIDUALS WITH DIFFERENT BODY MASS INDEX IN THE UKRAINIAN POPULATION

Abstract

The objective was to analyze the association of rs4977574-polymorphic variants of the ANRIL gene with the development of acute coronary syndrome in individuals with different body mass index. Materials and methods. The venous blood of 429 people (234 patients with acute coronary syndrome and 195 people in the control group) was used for the study. Genotyping of patients by rs4977574-polymorphic variants of the ANRIL gene was performed by real-time polymerase chain reaction (Real-time PCR) in the presence of TaqMan assay C_31720978_30. Statistical analysis of the results of the study was performed using SPSS software (version 17.0). Results. The distribution of genotypes according to SNP rs4977574 of the ANRIL gene in the group of patients with ACS and the control group among individuals with BMI < 25 kg/m2 does not differ. Among patients with BMI 25 kg/m2 the genotype distribution of the rs4977574-polymorphic variant of the ANRIL gene was statistically significant (р = 0.035). In the group of patients with BMI > 25 kg/m2 according to recessive (Pobserv = 0.014; ORobserv = 1.876, 95 % СІ = 1.137–3.095) and additive (Рobserv = 0.014; ORobserv = 2.118, 95% СІ = 1.166–3.849) models of inheritance before making adjustment, people with G/G genotype had a double risk of acquiring ACS than carriers of the dominant allele. After the adjustment, corresponding models of inheritance had the same risk rate – for recessive model (Рadjust = 0.013; ORadjust = 1.951, 95% СІ = 1.149–3.313) and additive model (Рadjust = 0.026; ORadjust = 2.039, 95 % СІ = 1.087–3.826). Conclusions. Individuals with BMI > 25 kg/m2, which were carriers of G/G genotype had a 2 times higher risk to acquire ACS than the individuals with the dominant allele. Prospects for further research. Further research will be aimed at studying the impact of ANRIL polymorphism upon the risk of ACS development depending on other risk factors.