Abstract
We evaluated the antioxidant and antibacterial activity of hexnane, ethyl acetate, acetone, methanol, ethanol, and water extracts of the Quercus acuta leaf. The antioxidant properties were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power, and total phenolic content. Antibacterial activity was assessed against general infectious pathogens, including antibiotic-resistant clinical isolates. The methanolic extract showed the highest DPPH radical scavenging activity and total phenolic content, while the reducing power was the highest in the water extract. The ethyl acetate extract showed the best antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) strains. Additionally, it displayed antibacterial activity against Staphylococcus aureus KCTC1928, Micrococcus luteus ATCC 9341, Salmonella typhimurium KCTC 1925, Escherichia coli KCTC 1923, and eight MRSA strains. These results present basic information for the possible uses of the ethanolic and ethyl acetate extracts from Q. acuta leaf in the treatment of diseases that are caused by oxidative imbalance and antibiotic-resistant bacterial infections. Six active compounds, including vitamin E, which are known to possess antioxidant and antibacterial activity, were identified from the extracts. To the best of our knowledge, this is the first study that reports the chemical profiling and antibacterial effects of the various QA leaf extracts, suggesting their potential use in food therapy or alternative medicine.
Highlights
Quercus acuta (QA) is widely distributed in the southern part of Korea, China, Japan, and Taiwan [1]
We identified the active substances in the QA leaf extracts using the Gas chromatography-mass spectrometry (GC-MS)
The active constituents were identified as cinnamic acid, phytol, α-linolenic acid, α-tocopherol, β-sitosterol, β-amyrin, and friedelin-3-ol from the hexane, ethyl acetate, and acetone extracts
Summary
Quercus acuta (QA) is widely distributed in the southern part of Korea, China, Japan, and Taiwan [1]. Only a few studies have investigated the pharmacological activity of various QA extracts and its active constituents. 3,5-di-O-galloyl protoquercitol, have been reported to possess antibacterial effect against both gram positive and gram-negative bacteria [3]. To the best of our knowledge, there have been no studies on the antibacterial effects of QA leaf extracts, which warrants further investigation. We prepared various extracts of the QA leaf using hexane, ethyl acetate, acetone, ethanol, methanol, and water in order to determine the optimal extraction conditions with respect to biological activity and phytochemical profiles. The antioxidant and antibacterial activities of the optimized QA leaf extracts were examined. The antibacterial activity was confirmed using the minimum inhibitory concentration (MIC) test against general infectious bacteria and antibiotic-resistant strains of clinical origin
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
