Analysis of synonymous codon usage bias in Ancylostoma ceylanicum

Abstract

Codon usage bias (CUB) is a species-specific phenomenon where some codons are used more commonly than their synonymous partners. It helps to understand the effect of recombination on the evolution of genomes, gene discovery, transgene design and much more. Here, we have analyzed the nucleotide composition and CUB of Ancylostoma ceylanicum protein-coding sequences. Genes are AT-biased and have a narrow GC3 distribution. Analysis of parity rule 2 indicates mutation pressure while analysis of neutrality plot confirms the influence of both the evolutionary factors on codon usage pattern. Codon context analysis revealed the skewed use of certain homologous codon pairs. The lower values of codon usage bias that are acquired as a result of mutation and selection pressure may be involved in higher transcriptional adaptation for living in diverse host conditions.