Abstract
Cell cultures have been obtained in vitro from a plant producing ecdysteroids, Chenopodium album. The composition of free and esterified sterols was compared in plant and cell cultures after purification by TLC and identification by GC/MS. Free sterols were the most abundant. A selectivity of sterol esterification was noted for 4,4-dimethylsterols (except in roots) and Δ7-sterols. The 4-desmethylsterols from leaves, roots and cell cultures of Chenopodium album were a mixture of Δ7-sterols (>61%) and Δ5-sterols (>36%). The Δ7-sterols were 24-ethylcholesta-7,22-dien-3β-ol and 24-ethylcholest-7-en-3β-ol. The isolated Δ5-sterols were sitosterol and stigmasterol. Traces of cholesterol and Δ5,7-sterols were also detected. Our attention was also given to ecdysteroid contents, which showed much lower quantity in cell cultures than in plants, confirming however the presence of 20-hydroxyecdysone.
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