Abstract
Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v). The inverse of value of retention factor () versus concentration of micelles () relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle ()—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) () were determined by the use of Foley’s equation.
Highlights
Micellar liquid chromatography (MLC) is a mode of conventional reversed-phase liquid chromatography which uses a surfactant solution above the critical micellar concentration as a mobile phase [1, 2]
Acetonitrile at the concentration of 20% v/v was utilized as an organic modifier
There are many agents in MLC which have an effect on retention such as [5, 37]: (i) type of organic modifier, (ii) concentration of organic modifier, (iii) concentration of surfactant, (iv) pH of mobile phase
Summary
Micellar liquid chromatography (MLC) is a mode of conventional reversed-phase liquid chromatography which uses a surfactant solution (anionic, cationic, or nonionic) above the critical micellar concentration (cmc) as a mobile phase [1, 2]. Some biogenic amines (adrenaline, dopamine, octopamine, 2-phenylethylamine, tyramine, and histamine) were determined. Dopamine is available as an intravenous medication acting on the sympathetic nervous system, producing effects such as increased heart rate and blood pressure It is involved in the regulation of a variety of functions, including locomotor activity, emotion and affect, and neuroendocrine secretion [19]. Due to biogenic amines are a large group of naturally occurring biologically active compounds, which are present in food products and can act as hormones and neurotransmitters, there are many different analytical techniques for their quantitative and qualitative determination. Paleologos and coworkers successfully separated nine biogenic amines (cadaverine, tyramine, putrescine, agamatine, spermidine, tryptamine, phenylalanine, spermine, and histamine) in the form of benzoyl derivatives by the use of MLC with gradient elution. Other biogenic amines (such as tyramine and tryptamine and their precursors: thyrosine and tryptophan) were determined in MLC systems in vine samples [36]
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