Abstract
In order to identify S-genotypes for improved pollination of pears, we attempted to determine the genotypes by analysis of protein polymorphism. Stylar proteins of European pears ( Pyrus communis) and Chinese pears ( Pyrus ussuriensis) were surveyed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). For both species, varietal differences were detected in a group of stylar proteins, which had similar M r values and p I values to those of the self-incompatibility-related ribonucleases (S-RNases) of the Japanese pear ( Pyrus serotina). All of these stylar proteins tested had RNase activity and reacted with an antiserum raised against the S 4-RNase of Japanese pear. Their N-terminal amino acid sequences showed a high level of homology with the S-RNases of Japanese pear. These results indicated that they are S-RNases and the products of S-alleles having RNase activity. In this paper we propose S-RNase types for eight European and eight Chinese pears. On the basis of the analyses the S-genotypes of the cultivars are discussed in comparison with past data from compatibility tests.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call