Analysis of Same Selected Immunomodulatory Properties of Chorionic Mesenchymal Stem Cells

Darina Bačenková,Lukáš Zachar,Gabriela Ižaríková,Radovan Hudák,Jozef Živčák,Marianna Trebuňová,Katarína Šurínová

doi:10.3390/app10249040

Abstract

Mesenchymal stem cells (MSCs) represent a population of adherent cells that can be isolated from multiple adult tissues. MSCs have immunomodulatory capacity and the ability to differentiate into many cell lines. Research study examines the immunomodulatory properties of MSCs isolated from chorion (CMSCs). Following the stimulation process, it was found that MSCs are capable of immunomodulatory action via the release of bioactive molecules as well as through direct contact with the immune cells. Immunomodulatory potential of the CMSCs was analyzed by modifying proliferative capacity of mitogen-activated lymphocytes. CMSCs and lymphocytes were tested in cell-to-cell contact. Lymphocytes were stained with carboxyfluorescein diacetate succinimidyl ester. Inhibition of the proliferation of activated lymphocytes was observed. Following the co-cultivation, the expression of markers involved in the immune response modulation was assessed. Afterwards, an increase in CMSCs expression of IL-10 was detected. Following the co-cultivation with activated lymphocyte, adhesion molecules CD54 and CD44 in the CMSCs increased. An increase of CD54 expression was observed. The properties of CMSCs, adherence and differentiation ability, were confirmed. The phenotype of CMSCs CD105+, CD90+, CD73+, CD44+, CD29+, CD45−, CD34−, CD54+ was characterized. It was demonstrated that chorion-derived MSCs have important immunomodulatory effects.

Highlights

Mesenchymal stem cells (MSCs) form a population of adherent cells with a characteristic phenotype in the absence of hematopoietic markers [1]
We tested the effect of activated chorionic mesenchymal stem cells (CMSCs) on lymphocytes proliferation
We focused on Thecharacterization main objective of of themorphology experimental and workthe wasdetermination to observe the immunomodulatory properties the detailed of the CMSCs phenotype

Summary

Introduction

Mesenchymal stem cells (MSCs) form a population of adherent cells with a characteristic phenotype in the absence of hematopoietic markers [1]. MSCs were first described by Friedenstein as fibroblast-like cells in the bone marrow [2]. In addition to bone marrow, adult MSCs were isolated from adipose tissue, liver and muscle tissue [3]. MSCs are characterized by their adherence to a plastic surface, fibroblast-like bipolar shaped cells morphology and a broadly defined phenotype. The expression of typical surface features for the MSCs population is as follows: CD105+, CD90+, CD73+, CD45−, CD34−, HLA-DR-. The MSCs have the following properties: CD9+, CD29+, CD44+, CD49+, CD54+, CD61+, CD63+, Appl.

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