Analysis of related factors of coins foreign bodies crossing the esophagus in 204 cases of children

Abstract

Objective:To explore the anti-tumor effect of Stat3 antisense oligodeoxynucleotide on human Hep-2 cell tumor-bearing BALB/c nude mouse, to study the therapeutic value of Stat3 antisense oligodeoxynucleotide on laryngeal cancer. Method:Hep-2 cells from human laryngeal carcinoma in logarithmic phase were inoculated subcutaneously into BALB/c mice to establish a model of human Hep-2 cell tumor-bearing BALB/c mice. They were divided into blank group, control group and different concentrations of AS3 groups(1 group, 2 group, 3 group, 4 group), and then intraperitoneally administered once a day for 4 weeks, measuring body weight twice a week, and the long and short diameters of the tumors were recorded. After 4 weeks, the mice in each group were weighted. The subcutaneous transplanted tumors were dissected, weighted, and inhibitory rate was obtained.Result:Stat3 antisense oligodeoxynucleotide can obviously inhibited the growth of human Hep-2 cell tumor-bearing BALB/c mice with the concentration of antisense oligodeoxynucleotide heightened. The IR in different concentration AS3 groups was obviously higher than that in the control group(P<0.01) .Conclusion:Stat3 antisense oligodeoxynucleotide can obviously inhibit the growth of subcutaneous transplanted tumors in human Hep-2 cell tumor-bearing BALB/c mice, and may have therapeutical effect on laryngeal cancer.