Abstract
Our work aims to explore Raman spectroscopy (RS) to study the effects of total protein of nasopharyngeal carcinoma (NPC) cell line CNE2 treated by different doses of X-ray irradiation. The total protein is extracted from the cell line after irradiation with no incubation (0 h) and with incubation for 72 h, respectively. Both paired-samplesttest and support vector machine (SVM) are employed for statistical analysis of Raman spectrum. The results show that several Raman bands assigned to total protein can be detected; and the X-ray irradiation have various effects on different components in total protein, especially for the dose higher than 6 Gy, and also associated with the incubation time after irradiation. The differences of content between 0 h groups and 72 h groups appear at bands of C–S stretching, C–H stretching, O=C–O−stretching and L-phenylalanine. It indicated no significant diversification on structures of protein, while the content may be varied during irradiation. Our spectroscopic results reveal that RS analysis of total protein of cell line irradiated by X-ray, in conjunction with mathematical statistical model, can be a potential method to explore the Raman characterization target of cell radiosensitivity, and then for making known the mechanism of radiosensitivity of tumor further.
Highlights
Radiotherapy is a primary treatment for nasopharyngeal carcinoma (NPC), and its efficacy is affected by the tumor in-house’s radiosensitivity, host status and the radiation injury of normal tissue
Ou et al / Analysis of protein from nasopharyngeal carcinoma cell line CNE2 irradiated by X-ray the investigation on the changes of intracellular biomacromolecules due to X-ray irradiation effects has become one of main methods to study tumor radiosensitivity [8,15]
The human NPC cell line CNE2 (Fujian Provincial Tumor Hospital, Fuzhou, China) are cultured in RPMI-1640 medium supplemented with 100 U/ml streptomycin, 100 U/ml penicillin and 10% Newborn
Summary
Radiotherapy is a primary treatment for nasopharyngeal carcinoma (NPC), and its efficacy is affected by the tumor in-house’s radiosensitivity, host status and the radiation injury of normal tissue. Ou et al / Analysis of protein from nasopharyngeal carcinoma cell line CNE2 irradiated by X-ray the investigation on the changes of intracellular biomacromolecules due to X-ray irradiation effects has become one of main methods to study tumor radiosensitivity [8,15]. As a method to acquire accurate frequency of molecular vibration, Raman spectroscopy allows noninvasive and nondestructive detection of changes of polypeptide and protein. RS discloses the fingerprint information about molecular vibrations which are significant to researchers [5,9,10,13]
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