Analysis of pork meat using loop mediated isothermal amplification (LAMP) to confirm halal status

Abstract

A method has been developed for identifying species from pork samples by loop-mediated isothermal amplification (LAMP) analysis of the wild boar (Sus scrofa) mitochondrial DNA D-loop region. DNA was extracted from raw and cooked pork and beef meat and subjected to polymerase chain reaction (PCR) and LAMP. Approximately 185 base-pair PCR products were found in the pork meat samples after amplification of the D-loop region. LAMP products only showed a smear band with the pork samples. Moreover, by adding CuSO4 to the LAMP product, the white Cu(OH)2 precipitate, which formed a ring-shaped deposit, was not seen in the pork samples, but was seen in the beef and negative control samples. Our finding showed the LAMP technique to be a powerful molecular-biological method for species identification. The addition of CuSO4 to the LAMP product is an alternative method that can be used in field studies, and does not require any gelelectrophoresis apparatus.