Abstract
Ground orchid (Spathoglottis plicata Bl) is an ornamental plant widely appreciated by the public because it has a beautiful shape, colour and flower formation. One of trigger decrease of S. plicata production caused by Fusarium wilt which is caused the Fusarium oxysporum. Using resistant S. plicata cultivars are expected to be an alternative to control the disease. A resistant S. plicatta plantlet to Fo has been initiated by in vitro selection on medium countaining fusaric acid on different concentrations. This study aims to determine the peroxidase activity and total phenol of S. plicata plantlet. Research conducted at the in vitro Laboratory, Department of Biology, Faculty of Mathematics and Natural of Sciences, University of Lampung. The study design was Completely Randomized. Data analyzed using Analysis of Variance and if significantly different followed by Least Significant Difference (LSD) test 5% significance level. The results showed that increasing the concentration of fusaric acid, it also increases the peroxidase enzyme activity and total phenol in plantlet that resistant to F. oxysporum. At a concentration of 40 ppm, peroxidase activity and the highest total phenol content are 0.536 unit/mg/second and 10.33%.
Highlights
The cultivation of orchids have many obstacles, one of which is fusarium wilt which is caused by Fusarium oxysporum [10]
Cultivars that are resistant to F. oxysporum infection can be identified by in vitro selection in a medium supplemented with fusaric acid [3]
Fusaric acid (5-n-butylpicolinic acid) is a non-specific mycotoxin produced by F. oxysporum that causes symptoms of wilt and rot in plants [11]
Summary
The cultivation of orchids have many obstacles, one of which is fusarium wilt which is caused by Fusarium oxysporum [10]. Fusaric acid (5-n-butylpicolinic acid) is a non-specific mycotoxin produced by F. oxysporum that causes symptoms of wilt and rot in plants [11]. Using selectors fusaric acid as the agent in vitro selection may produce mutant cell or tissue that is insensitive to fusaric acid, after being regenerated into plants can produce strains that are resistant or tolerant to pathogen infection. This method had been carried out among others on the plant plantlets vanilla [9], showed resistance to fusarium wilt.
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