Kandungan Klorofil Daun Planlet Anggrek Tanah (Spathoglottis plicata Blume.) Hasil Pengimbasan Ketahanan terhadap Asam Fusarat secara In Vitro

Abstract

Spathoglottis plicata cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum. Planlet S. plicata to the F. Oxysporum were,selected in the solid Vacin and Went (VW) medium was added with fusaric acid at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm). The aim of this research was to know the content of chlorophyll a, b, and total of leaves of planlet S. plicata. The research was carried out in January to March 2015 in the Laboratory of Tissue Culture, Departement of Biology, Faculty of MIPA, Lampung University. This study used a completely randomized design with 5 replications. Data were analyzed with the variance (Anova) and if them different will be continued by LSD test performed at 5% significance level. Chlorophyll extraction and calculation method using Harbourne (1987) with the solvent used was 80% acetone. The absorbance was measured with a spectrophotometer (Shimudzu UV 800) at wavelength (I») of 646 nm and 663 nm. The result showed that the content of chlorophyll a, b, and the total on the leaves planlet S. plicata has increased significantly on the concentration of the fusaric acid 10 ppm, 20 ppm, 30 ppm, and 40 ppm compared with controls. Increased of chlorophyll content showed that plantlet S. plicata was resistant to the fusaric acid and expected to be resistance to F.oxysporum.Key words: Spathoglottis plicata, Fusarium oxysporum, Fusaric acid, and Chlorophyll