Abstract
Abstract High-performance liquid chromatography using a hydrophilic hard-gel TSK-G3000SW column was employed to investigate the peptic fragmentation of a commercial human immunoglobulin (IgG) and its components, i.e., monomeric, dimeric, and aggregated IgG. The monomeric IgG was digested to yield two major fragments, F(ab′)2 and pFc′, and at least six minor fragments. The dimeric IgG was digested to give three major fragments including F(ab′)2, pFc′, and a fragment similar to F(ab′)2 dimer. The main product from the aggregated IgG was an unknown fragment, and the F(ab′)2 fragment was scarcely observed. These observations demonstrated that three IgG components were different from each other in the susceptibilities to peptic digestion and also suggested that the dimer and aggregates were associated mainly through their Fab regions. The TSK-G3000SW column was very useful as a means of fractionating monomer, dimer, and aggregates of IgG as well as a potential means of analyzing the peptic fragment of IgG.
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