Abstract

In this study, the utral-performance liquid chromatography (UPLC) method with UV-PDA was utilized to develop a rapid, sensitive reliable method for analysis of 5 penicillin antibiotics in cosmetics productions which the presence of these penicillin in commercial cosmetics samples is prohibited. The samples was prepared by liquid-liquid extraction and then the analyses was carried out on UPLC-PDA. The chromatographic column used was Acquity UPLCTM BEH C18 (100 mm ×2.1 mm,1.7μm) and the mobile phase is Acetonitrile and potassium dihydrogen phosphate(PBS) Buffer solution pH 5.5. Chromatographic separation was obtained by gradient elution.

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