Abstract
Background"Core microbes" play a key role in the development of caries and lead to microbial disorders. Our goal was to detect the core microbes associated with the microbiota imbalance in early childhood caries (ECC).MethodsFifteen caries-free children and fifteen high-caries (DMFT ≥ 10) children aged 4–6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from samples of plaque in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.ResultsThere were statistically significant differences between the groups regarding the relative abundance of Streptococcus mutans (Wilcoxon rank-sum test, P < 0.05). No significant difference was found between the groups regarding other species or functional genes.ConclusionS. mutans, together with other pathogens, may play a prominent role and act as "core microbes" in the occurrence and development of early childhood caries.
Highlights
Childhood Caries (ECC) is defined as one or more decayed, missing or filled surfaces presenting in any deciduous tooth of a child under six years old [1]
Our aim was to detect pathogenic bacteria that were related to the microbial dysbiosis of Early Childhood Caries (ECC), providing a theoretical basis for caries prevention and therapy
Bacterial community structure characteristics based on the Bray–Curtis distance We converted the Operational taxonomical units (OTUs) relative abundance table into a relative abundance table of taxonomic level from phylum to genus
Summary
Childhood Caries (ECC) is defined as one or more decayed (non-cavitated or cavitated lesions), missing or filled (due to caries) surfaces presenting in any deciduous tooth of a child under six years old [1]. Surveys have made it clear that, except for the prevalence of ECC, it is largely untreated in children under the age of three [2]. The caries rate of deciduous teeth in China is 71.9% in 5-year-old children, According to the quadruple factors theory of caries, bacteria, food, host and time are all necessary conditions for caries occurrence. Dental caries have been defined as microbial dysbiosis [4]. For the microbial factors of dental caries, it is generally accepted that microorganisms ferment carbohydrates in dental plaque to produce acid, which leads
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