Abstract

This study aims to determine the microbiological contamination in the main water source of the Muara Nusa Dua Reservoir, the Tukad Badung River, and to determine the microbiological quality of fresh Oreochromis niloticus caught in the Muara Nusa Dua Reservoir in Denpasar. The study was conducted in May-August 2019 at the Denpasar Veterinary Laboratory. The method used is a descriptive method that aims to describe or describe the condition of the Tukad Badung watershed which empties into the Muara Nusa Dua Reservoir (Estuary Dam). Objective tests include the total bacterial test, the Coliform test, and the Escherichia coli contamination test. Water quality measurements are also carried out as supporting data from this study. The results of the study stated that there was microbiological contamination in the water source of the Muara Nusa Dua Reservoir, the Tukad Badung river, which also had an impact on the waters of the Muara Nusa Dua Reservoir. Microbiological contamination research results showed the microbiological quality of ten samples of Oreochromis niloticus in the form of the highest total bacteria was at 2.2x104, the highest total Coliform was 1.9x104 and the highest Escherichia coli contamination was at 93 APM / g with an average reservoir water quality Muara Nusa Dua in the form of temperature 27.3 °C, salinity 0.208 ‰, degree of acidity (pH) 6.5, dissolved oxygen (DO) 5,97 ppm. Organoleptic observations included the appearance, smell, and texture of Oreochromis niloticus with good results because when testing Oreochromis niloticus is still alive

Highlights

  • The use of common-pool natural resources tends to create conflicting interests in the community

  • The results of this study are observations regarding the level of contamination and the quality of pathogenic microbial contamination, namely total bacteria, total Coliform and Escherichia coli in reservoir water sources, and Oreochromis niloticus fish caught by fishermen in Muara Nusa Dua Reservoir

  • Water samples are measured for water quality. after the field activities are finished, water and Oreochromis niloticus samples are stored in a styrofoam box and immediately taken to the laboratory by road within 15 minutes of travel and the fish are still alive when they arrive at the laboratory for microbial contamination testing in the form of total bacteria, total coliforms, and E. coli

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Summary

Introduction

The use of common-pool natural resources tends to create conflicting interests in the community. One form of common-pool resource is a reservoir, which is a form of inland water beside rivers and lakes. Indonesia has several reservoirs scattered in each region, one of which is the Muara Nusa Dua Reservoir which is located in Pemogan Village, South Denpasar District, Bali Province. It has a 35hectare puddle area with a depth of approximately four meters during normal conditions and can reach a depth of five meters at high tide. Local people often use this reservoir area as a place of worship, fishing, recreation, and trading. There are several types of fish caught, ranging from surface fish such as Oreochromis niloticus, Oreochromis

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