Abstract
A liquid chromatographic method was developed to resolve a comprehensive set of metabolites of JWH-018 and JWH-073. In addition to the chromatographic analysis method, an extraction method was developed to recover a broad range of synthetic cannabinoid metabolites, including carboxylic acid metabolites that are not traditionally recovered using a high pH liquid/liquid extraction. The extraction and analysis methods were used to identify and quantify the significant metabolites in urine samples. The chromatographic method detailed in this application note employed a RP-HPLC column and MS/MS detection. The quantitative range validated for all metabolites was 1 ng/mL to 500 ng/mL in urine. Based on the data, this method is suitable for quantifica- tion of metabolites of JWH-018 and JWH-073 to support broader research studies that positively identify clinically significant metabolites and their concentrations in urine.
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