Analysis of lymphocyte subsets and interleukin-2 receptor in patients with severe fever with thrombo-cytopenia syndrome in Zhoushan island

Abstract

Objective To analyze the characteristics of lymphocyte subsets and interleukin-2 re-ceptor （CD25） in patients with severe fever with thrombocytopenia syndrome （SFTS） in Zhoushan island. Methods Automated blood analyzer , automatic biochemical analyzer and flow cytometry were used to meas-ure hematological parameters , biochemical parameters and lymphocyte subsets in patients with SFTS before and after treatment .The dynamic changes and correlation analysis were statistically analyzed .Results The counts of white blood cells （WBCs）, platelets, total T cells and CD4＋T cells in patients with SFTS before treatment were significantly lower than those in healthy subjects .The counts of WBCs and platelets were sig-nificantly increased after ten days of treatment , and the level of CD25＋cells was higher than that in control group, but the level of total T cells and CD 4＋T cells were still lower than those in healthy subjects （ P〈0.05）.Patients died of SFTS had significantly lower counts of total T cells , CD4＋T cells, CD8＋T cells and CD25＋cells than cured patients （P〈0.05）.The level of CD25＋cells and B cells were positively correlated with WBC level （P〈0.05）.Platelet level was positively correlated with the percentage of CD 4＋T cells （P〈0.05）.The level of alanine aminotransferase and creatine kinase were positively correlated with the number of CD3＋T and CD8＋T cells （P〈0.05）.Conclusion SFTS virus infection affected the percentage of body′s immune cells, especially the level of CD4＋T cells.The imbalanced immune system was correlated with the counts of WBCs , platelets and various enzymes in serum and it might be one of the factors causing extensive damage of multiple organs and tissues . Key words: Severe fever with thrombocytopenia syndrome; Severe fever with thrombocytopenia syndrome bunyavirus; Lymphocyte subset; Interleukin-2 receptor