Abstract
The current study was undertaken to analyze in vitro the functional activity of cells extracted from diseased periodontal tissues. Tissue was removed from 33 patients undergoing periodontal surgery. All patients had received repeated oral hygiene instruction and root planing prior to the surgery. The excised tissue was cut finely, digested in collagenase for 90 min, and then forced through a stainless steel grid to obtain a single cell suspension. The responses to various doses of PHA, Con A, and PWM were assessed in 2, 3, and 4‐day cultures using peripheral blood lymphocytes as controls. Blastogenesis was measured by the uptake of 3H‐thymidine.The extraction procedure resulted in a mean of 3.29 ± 0.29 × 105 lymphocytes per 100 mg of tissue. These cells had a mean viability of 76.8 ± 2.2%. Peripheral blood lymphocytes gave typical dose response curves for each of the three mitogens with a peak response occurring at 3 days. However, the lymphocytes extracted from the periodontal tissues failed to respond to any mitogen at any time. The failure to respond was not due to the effect of local anesthetic nor to the effect of collagenase pretreatment. These results may therefore indicate that the cells are maximally stimulated in vivo prior to extraction and/or there is production of suppressor factors in vitro.
Published Version
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