Abstract
The aim of this study was to analyze K-ras codon 12 mutation in the pancreatic tissue of Serbian patients with pancreatic cancer and assess whether the given mutation can be used as a molecular marker for this disease. The study was performed on pancreatic tissue samples obtained from 40 patients with clinical diagnosis of pancreatic cancer. The presence of K-ras codon 12 mutation was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Our study showed that K-ras mutation is present with a high frequency (66%) in the pancreatic tissue of patients with pancretic cancer.
Highlights
Pancreatic cancer is one of the most aggressive human malignancies and the fourth leading cause of cancer death in Europe and North America (Klapman and Malafa, 2008)
The aim of this study was to analyze the status of K-ras codon 12 mutation in the pancreatic tissue of Serbian patients with pancreatic cancer, investigate the correlation between the presence of the mutation and histopathological findings, and assess whether the presence of the mutation might be used as a molecular marker in future screening protocols for pancreatic cancer
Detection of K-ras codon 12 mutation by PCRRFLP analysis was successfully performed for all tissue samples
Summary
Pancreatic cancer is one of the most aggressive human malignancies and the fourth leading cause of cancer death in Europe and North America (Klapman and Malafa, 2008). Patients with this tumor have a median survival time of 6 months after initial diagnosis and a 5-year survival rate of only 2% (Tomaino et al, 2007). These facts make pancreatic cancer extremely difficult to treat. An understanding of the molecular basis of transformation into a malignant tumor of the pancreas may provide a basis for the development of more effective strategies for the prevention, diagnosis, and treatment of this cancer
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