Abstract
The research is supported by a Project Funded by the brand professional project form Yancheng teachers college (2016) and the teaching reform and practice of medicinal chemistry (2018YCTUJGY006) and the innovation and entrepreneurship training program for college students in jiangsu province （ 201910324016Z ） Abstract The content of ammonia nitrogen in water was determined by national standard method - nessler's reagent spectrophotometry. Through comparison, it was found that the method of reagent configuration, the amount of reagent, the detection wavelength, the color development time and the temperature had an impact on the analysis results in the ammonia nitrogen measurement in water by the reagent spectrophotometry. Based on the experimental results, the factors influencing the determination of ammonia nitrogen by spectrophotometry with nessler's reagent were discussed. Keywords Nessler's reagent spectrophotometry, Ammonia nitrogen, Influencing factors, Blank value, ABS value, Correlation coefficient. DOI : 10.7176/JEP/10-31-08 Publication date: November 30 th 2019
Highlights
Ammonia nitrogen in aqueous solution is nitrogen in the form of free ammoniaor ionic ammonia (NH4+)
The commonly used method for the determination of ammonia nitrogen in environmental monitoring laboratories in China is nessler's reagent spectrophotometry, which has the characteristics of simplicity, rapidity, economy and high benefit
In this paper, the blank value, Abs value and correlation coefficient of the reagent photometry were analyzed, and the optimal conditions were determined through comprehensive consideration, so as to achieve the optimal expected effect
Summary
Ammonia nitrogen in aqueous solution is nitrogen in the form of free ammonia (or non-ionic ammonia, NH3)or ionic ammonia (NH4+). Especially fish, are sensitive to non-ionic ammonia. To protect freshwater aquatic organisms, the concentration of non-ionic ammonia in water should be less than 0.02 mg L-1. The commonly used method for the determination of ammonia nitrogen in environmental monitoring laboratories in China is nessler's reagent spectrophotometry, which has the characteristics of simplicity, rapidity, economy and high benefit. In actual operation, factors such as reagent allocation method, reagent dosage and temperature will affect the blank value, Abs value and correlation coefficient of ammonia nitrogen measurement. In this paper, the blank value, Abs value and correlation coefficient of the reagent photometry were analyzed, and the optimal conditions were determined through comprehensive consideration, so as to achieve the optimal expected effect
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