Abstract
Ultraperformance liquid chromatography coupled with negative electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) was used to determine 7 hydroxy fatty acids in the pollen of Brassica campestris L. var. oleifera DC. All the investigated hydroxy fatty acids showed strong deprotonated molecular ions [M–H]−, which underwent two major fragment pathways of the allyl scission and the β-fission of the alcoholic hydroxyl group. By comparison of their molecular ions and abundant fragment ions with those of reference compounds, they were tentatively assigned as 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (1), 10,11,12-trihydroxy-(7Z,14Z)-heptadecadienoic acid (2), 7,15,16-trihydroxy-9Z,12Z-octadecadienoic acid (3), 15,16-dihydroxy-9Z,12Z-octadecadienoic acid (4), 15-hydroxy-6Z,9Z,12Z-octadecatrienoic acid (5), 15-hydroxy-9Z,12Z- octadecadienoic acid (6), and 15-hydroxy-12Z-octadecaenoic acid (7), respectively. Compounds 3, 5, and 7 are reported for the first time.
Highlights
Pollen contains many kinds of nutrient which are necessary to human body. It is well known as a natural nutrition, health food, and “perfect food.” e pollen preparation (Qian Lie Kang Tablets) made of Brassica campestris L. var
The pollen preparation has been used as phytotherapy for benign prostatic hyperplasia (BPH)
We investigated the fragmentation behaviors of hydroxy fatty acids in a Micromass Q/TOF Mass Spectrometer and emphasized on the structural determination of hydroxy fatty acids
Summary
Pollen contains many kinds of nutrient which are necessary to human body. It is well known as a natural nutrition, health food, and “perfect food.” e pollen preparation (Qian Lie Kang Tablets) made of Brassica campestris L. var.oleifera DC. has been widely used in China as a good treatment for benign prostatic hyperplasia (BPH), and a chemical study has found plenty of fatty acids in the pollen [1]. F 2: Total ion chromatogram of supercritical uid extract of pollen of Brassica campestris L. var.
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