Abstract
Forensic Entomogenetics deals with the application of entomology allied to molecular biology techniques, being a tool for obtaining evidence that can assist in the solution of crimes. An application of forensic entomogenetics is the identification of the genetic profile of an individual by DNA analysis of the digestive tract of a mosquito that has stung it and can be used in cases of kidnapping and captivity. The main objective of this work was to expose a group of mosquitoes of the genus Aedes to the human blood (donor) and to observe if after 24h it is possible to detect traces of the human DNA that serves to identify the individual. For this, a group of 20 specimens was separated at two times, 1h and 24h after the blood supply, for DNA extraction using the QiAmp DNA Investigator kit (Qiagen). Human DNA concentration analysis was determined by Nanodrop 2000/2000c spectrophotometer (Thermo Fisher Scientific), and the PCR amplification of this human mitochondrial marker material was positive. The feasibility of the tested process for obtaining DNA and human-specific marker amplification from mosquitoes hás been confirmed and it is hoped to contribute to research actions within the forensic sciences.
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