Abstract
Objective To investigate the infection of 23 kinds of human papillomavirus (HPV) subtypes in female cervical epithelium samples and to analyze their relationships with age and results of cytologic test in Haikou area. Methods A total of 4 037 local healthy women were enrolled in this study from July 2013 to August 2016, 1 967 of whom received cervical cytology test. Cervical cell samples collected from those women were detected for HPV typing by using PCR-reverse dot blot hybridization. Results (1) The total positive rate of HPV in 4 037 samples was 22.15% (894 cases), and the detection rates of carcinogenic, possibly carcinogenic and non-carcinogenic HPV were 16.13%, 3.99% and 5.55% (651, 161 and 224 cases), respectively. The positive rates of 6 genotypes were high, which were HPV52, 53, 81, 51, 16 and 58 in turn. (2) The detection rates of carcinogenic, possibly carcinogenic HPV and some of the genotypes (HPV18, 52, 53, 66) increased with age (all P<0.05). (3) Multiple infection in HPV-positive women accounted for 24.38% (218/894), in which the infection rates of carcinogenic types declined with age and the numbers of HPV genotypes in carcinogenic infections were negatively correlated with age (both P<0.05). (4) Only 2.49% (49/1 967) of the samples were positive for cervical cytologic test, classified into the ≥ASC-US (atypical squamous cells of undetermined significance) group. The detection rates of eight kinds of carcinogenic and two kinds of possibly carcinogenic HPV in ≥ASC-US group were significantly higher than those in the negative (no intraepithelial lesion or malignant lesion, NILM) group (all P<0.05). Conclusion This study indicates that Haikou women have higher rates of HPV infection, especially the elderly group. HPV subtype infections present some regional characteristics and are mainly single type infection. Cervical cancer screening should be combined with tests for HPV and cytology analysis to improve its effectiveness. Key words: Human papillomavirus; PCR-reverse dot blot hybridization; Genotyping; Multiple infection; Liquid based cytology
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