Abstract
A method for estimating Nɛ-(ß-l-aspartyl)-l-lysine, Nɛ-(γ-l-glutamyl)-l-lysine and the usual protein amino-acids within 4 hours is given, using a commercial amino-acid analyser, common ion-exchange resin, and modified sodium citrate buffers. If these isopeptides are to be estimated alone a new accelerated procedure can be used employing 0.21 N sodium citrate buffer, pH 3.52. Isopeptide contents of some fractions from heated ribonuclease, heated monomer, dimer, and oligomers, are given. Ribonuclease dimer contains both isopeptides adding up to 0.8 mole of isopeptide residue per mole of dimer.
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