Abstract
Employing polyacrylamide gradient-gel electrophoresis, the genetic control of leaf glutamate oxaloacetate transaminase (GOT) isozyme variants and their linkage to ds1 (desynapsis), y (tuber flesh colour), cr (crumpled) and yc (yellow cotyledon) were studied in diploid tuberous Solanum species hybrids. Leaf GOT isozymes proved to be dimeric and under the control of two independently segregating loci (Got-1 and Got-2) with two (1 s , 1 f ) and three (2 s , 2 f , 2 n )alleles respectively. The 2 n allele was concluded to encode a 2 fallozyme subunit capable of forming heterodimeric but no homodimeric active molecules. As expected with Got-1 and Got-2 isozymes being localized in different cellular compartments, no intergenic heterodimer formation was observed. In contrast to earlier studies, a gradual rather than discrete transition between the tuber flesh colour classes white and yellow was observed in segregating progenies. Adopting a standardized classification method, however, monogenic recessive inheritance of white tuber flesh was confirmed. Got-1, ds1 and cr were found to represent a single linkage group. Among the presently studied marker loci no further linkages were detected. As to linkage analysis, the difficulties imposed by gametophytic incompatibility in nearly all diploid potato species and by the frequent occurrence of recessive (sub)lethal genes are discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.